Antiproliferative Activity of Triterpenoid and Steroid Compounds from Ethyl Acetate Extract of Calotropis gigantea Root Bark against P388 Murine Leukemia Cell Lines

Calotropis gigantea has been known to produce bioactive secondary metabolites with antiproliferative activities against cancer cells. Herein, we extracted the using ethyl acetate from its root bark and further tested P388 murine leukemia cell lines. The subfractions extract was obtained Vacuum Liquid Column Chromatography (VLCC), followed by Gravity (GCC). subfraction C2 D1 were identified contain triterpenoids steroids most potent cytotoxicity Brine Shrimp Lethality Test (BSLT). A 3-(4,5-dimethylthiazol-2-yl) -2-5 diphenyl tetrazolium bromide (MTT) assay suggested that highest lines (IC50 = 21.79 μg/mL), as opposed 50.64 µg/mL) 49.33 µg/mL). compound in are taraxerol calotropone, respectively. Though calotropone active inhibiting leukemic lines, their IC50s lower than extract, which is probably due synergism of metabolites.